New transcriptomes for putative members of Longamoebia. 'Longamoebia'

Longamoebia is one of the most morphologically diverse clades of Amoebozoa. The taxonomy and phylogeny of Longamoebia is poorly understood partly due to the growing number of molecular studies that report unsuspected affiliations of lineages with extremely different morphotypes in the group. In this study, we conducted a more comprehensive phylogenomic analysis including all of putative members of Longamoebia to assess its monophyly. Our phylogenomic analysis yielded a well-resolved tree of Amoebozoa and highly supported novel relationships. Our study strongly demonstrates that Longamoebia sensu Smirnov et al. (2011) is not monophyletic. Thecamoebida forms a strongly supported sister group relationship with clade Flabellinea (Dactylopodida and Vannellida), while Dermamoebida (Mayorella + Dermamoeba) form an independent branch basal to other members of Discosea. The remaining groups including members of Centramoebida form a consistently well-supported clade that was shown to form a sister group relationship with Himatismenida. This robust clade shares the unique cytoskeletal features of coiled cytoplasmic microtubule network and F-actin characters.