IL-3 receptor signaling suppresses chronic intestinal inflammation by controlling mechanobiology and tissue egress of regulatory T cells

RNA from unchallenged mouse Il3r-/- and Il3r+/+ thymocytes was isolated with the NucleoSpin RNA Kit for RNA isolation (Machery Nagel). RNA-Analysis and sequencing was performed by the Core unit next generation sequencing of the University Hospital Erlangen. The quality of isolated RNA was determined using an Agilent 2100 bioanalyzer with RNA 6000 Nano kit and related software (Agilent). Sequencing libraries were generated from RNA samples using the TruSeq Stranded mRNA Kit (Illumina). Libraries were sequenced on a HiSeq 2500 platform (Illumina). The reads were aligned with Star v2.5.4b against the GRCm38 reference genome. Reads were counted with featureCounts of the Rsubread package v2.8.1.