Single-cell RNA sequencing of mouse subcutaneous EO771 tumors to study synergistic effects of NEDD8 depletion and anti-PD-1 therapy

We have established the phenotype that depletion of NEDD8 in tumor cell has synergestic effect with anti-PD1 therapy in mouse EO771 subcutaneous tumor model. In this study we performed 10X Flex single cell RNAseq of mouse tumor samples to study the tumor intrinsic and immune environment changes that could explain the outcome.16 mouse (4 per group) were injected subqutaneously with WT or NEDD8 KO EO771 breast cancer cell line, followed by i.p anti-PD1 treatment or IgG2a control treatment for every three days after tumor establishment. Tumors were collected on day 19 after cancer cells injection and three days after the last anti-PD1 treatment for scRNAseq.