Feeding experiment microbiome - fungi

Shifts in microbiome community composition can have large effects on host health. It is therefore important to understand how perturbations, like those caused by the introduction of exogenous chemicals, modulate microbiome community composition. In poison frogs within the family Dendrobatidae, the skin microbiome is exposed to the alkaloids that the frogs sequester from their diet and use for defense. Given the demonstrated antimicrobial effects of these poison frog alkaloids, these compounds may be structuring the skin microbial community. To test this, we first characterized microbial communities from chemically defended and closely related non-defended frogs from Ecuador. Then we conducted a laboratory experiment to monitor the effect of the alkaloid decahydroquinoline (DHQ) on the microbiome of a single frog species. In both the field and lab experiments, we found that alkaloid-exposed microbiomes are more species rich and phylogenetically diverse, with an increase in rare taxa. To ..., , , # Oophaga sylvatica experimental microbiome - fungi [https://doi.org/10.5061/dryad.4mw6m90hn](https://doi.org/10.5061/dryad.4mw6m90hn) This data was generated in a controlled feeding experiment to test for the role of the alkaloid decahydroquinoline (DHQ) in shaping the skin microbiome of the poison frog Oophaga sylvatica. Samples were collected in a time series from animals that were in the experimental group (DHQ-fed) and control group (vehicle control fed). Metadata can also be found as an uploaded csv data file. The variables of which are: * **SampleName**: Sample Name that corresponds to FASTA files * **PlateNumber**: 96 well plate used for library prep * **WellNumber**: Well number within the 96 well plate for library prep * **SampleID**: Tracking number for library prep processing * **FrogSpecies**: Either AF (Allobates femoralis) or OS (Oophaga sylvatica) * **FrogNumber**: Frog identifier used throughout feeding experiment. Box designate samples of the frog housing that were...