Multi-racial cytokine profiling reveals immune dysregulation, not chronic inflammation, in polycystic ovary syndrome

This dataset contains plasma concentrations of 96 immune-related proteins measured using a custom Luminex xMAP assay in 40 women (20 with PCOS, 20 controls) matched for age, BMI, and race (Black and White). The comprehensive panel includes:
Growth Factors (12): EGF, FGF-2, FLT3L, G-CSF, GM-CSF, M-CSF, PDGF-AA, PDGF-AB, SCF, TGF-α, TPO, and VEGF-A
Cytokines (35): APRIL, BAFF, IFN-α2, IFN-β, IFN-γ, IFN-ω, IL-1α, IL-1β, IL-1Rα, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12p40, IL12-p70, IL-13, IL-15, IL-16, IL-17A, IL-17E, IL-17F, IL-18, IL-20, IL-21, IL-22, IL-23, IL-24, IL-27, IL-28A, IL-29, IL-31, IL-33, IL-34, IL-35, LIF, TNF-α, TNF-β, and TSLP
Chemokines (41): Including 6CKINE, BCA1, CCL28, CTACK, CXCL16, ENA78, EOTAXIN variants, FRACTALKINE, GCP2, GRO-α, IP-10, MCP variants, MIP variants, RANTES, and others
Other Immune-Related Proteins (8): CD137, CD40L, Granzyme A, Granzyme B, Perforin, sFAS, sFASL, and TRAIL
, Study Participants and Cohort Design Samples were obtained from a prospective study of over 2500 women and adolescents (aged ≥14 years) presenting for evaluation of androgen excess at reproductive endocrinology clinics. PCOS was defined using the 1990 National Institutes of Health (NIH) criteria. For this analysis, we performed a cross-sectional case-control study with a subsampled cohort of 40 women: 20 with PCOS and 20 controls, matched for race (10 White, 10 Black in each group), age, and BMI. Fasting blood samples were obtained during the follicular phase (cycle days 3-8), and plasma was separated and cryopreserved.
Luminex Assay Plasma samples (10 μL) were analyzed using blinded Xmap Intelliflex Luminex assays. CRP was measured at 1:40,000 dilution, while 96 cytokines were measured without dilution using comprehensive custom immune protein panels: Human Cytokine/Chemokine/Growth Factor panel A (Cat# HCYTA-60K-PXBK48) and panel B (Cat# HCYTB-60K-PXBK48), and Human Cardiovascular Dis..., # Multi-racial cytokine profiling reveals immune dysregulation, not chronic inflammation, in polycystic ovary syndrome

Dataset DOI: [10.5061/dryad.ttdz08m9t](10.5061/dryad.ttdz08m9t)

## Description of the data and file structure

**Study Participants and Cohort Design** Samples were obtained from a prospective study of over 2500 women and adolescents (aged ≥14 years) presenting for evaluation of androgen excess at reproductive endocrinology clinics. PCOS was defined using the 1990 National Institutes of Health (NIH) criteria. For this analysis, we performed a cross-sectional case-control study with a subsampled cohort of 40 women: 20 with PCOS and 20 controls, matched for race (10 White, 10 Black in each group), age, and BMI. Fasting blood samples were obtained during the follicular phase (cycle days 3-8), and plasma was separated and cryopreserved.

**Luminex Assay** Plasma samples (10 μL) were analyzed using blinded Xmap Intelliflex Luminex assays. CRP was measured at 1:40,000 dilut..., This dataset contains completely de-identified human subjects data that complies with Dryad's standards for public domain publication. The original study was conducted under institutional review board approval at the University of Alabama at Birmingham and Cedars-Sinai Medical Center between 1987 and 2010. Written informed consent was obtained from all participants, including explicit consent for future research use of biological samples and associated de-identified data.

The dataset has been thoroughly de-identified and contains only the following variables: cytokine concentrations (96 analytes), self-reported race (White/Black), PCOS diagnosis status, BMI, and age. All direct identifiers have been removed, including participant codes, dates, geographic identifiers, and any other information that could potentially identify individual participants. No linking codes or keys exist that could re-identify participants.

The de-identification process ensures compliance with applicable priva...