Toward three-dimensional DNA industrial nanorobots

Nanoscale industrial robots have potential as manufacturing platforms, capable of automatically performing repetitive tasks to handle and produce nanomaterials with consistent precision and accuracy. We demonstrate a DNA industrial nanorobot, that fabricates a three-dimensional (3D) optically active, chiral structure from optically inactive parts. By making use of externally controlled temperature and ultraviolet (UV) light, our programmable robot, ~ 100 nanometers in size, grabs different parts, positions and aligns them so that they can be \"welded\", releases the construct and returns to its original configuration ready for its next operation. Our robot can also self-replicate its 3D structure and functions, surpassing single-step templating (restricted to two-dimensional (2D)) by using folding to access the third dimension and more degrees of freedom. Our introduction of multiple-axis precise folding and positioning as a tool/technology for nanomanufacturing will open the door to more..., , , # Toward three-dimensional DNA industrial nanorobots

[https://doi.org/10.5061/dryad.tdz08kq5h](https://doi.org/10.5061/dryad.tdz08kq5h)

## Description of the data and file structure

All data needed to support the conclusions of this manuscript are included in the main text or the supplementary materials. Raw data is available here.

1\. Fig_1D_lane78_20180712-4s-4.bip is the gel image to explain the mobility difference between 2D trimer and 3D robot trimer. The lane 8 and 9, from left, were used in the main text.
2\. Fig_1G_CD spectrum_left handed.xls is the data for the CD spectrum of figure 1G, left-handed. The column B was the CD spectrum of buffer alone, used as the baseline. The column C to Y were the CD intensity data for different samples. The column Z is the average data of the CD data from column C to Y. The data used to draft the plots in the main text was column AA, substration between the average and the baseline.
3\. Fig_1G_CD spectrum_right handed.xls is the data for th...