Whole human genome sequencing of an African male individual (HapMap: NA18507) using the Illumina HiSeq 2500 and paired 100 base reads

We have generated paired-end sequence data covering the genome of an African male individual to a sequence depth of more than 30-fold using the Illumina HiSeq 2500. This individual is a member of the population samples described in the PhaseI and PhaseII HapMap Projects and is from the Yoruba in Ibadan, Nigeria (abbreviation: YRI). The DNA identifier for this individual is NA18507. We obtained the DNA sample NA18507 from The Coriell Institute for Medical Research. Starting with 500ng of DNA, and following random fragmentation, we generated a PCR-Free sequencing library with a median insert size of ~300 bp. 100 base sequence reads were generated from both ends of these templates using the Illumina HiSeq 2500. We carried out purity-filtering (PF) to remove mixed reads, where two or more different template molecules are close enough on the surface of the flow-cell to form a mixed or overlapping cluster. No other filtering of the data has been carried out prior to submission.