Keratinocyte response to SNHG26 silencing

Analysis the transcriptome of keratinocyte after SNHG26 silencing with or without TNFa treatment for 3 hours. SNHG26 is a conseved lncRNA which is upregulated in the epidermis of human and mouse acute wound. Results of this profiling provide insight into the biological role of SNHG26 in keratinocytes. Human adult primary keratinocytes were cultured in Epilife medium supplement with Human Keratinocyte Growth Supplement and 1X Penicillin-Streptomycin. 10nM SNHG26-ASO or Ctrl_ASO was transfected for 24 hours followed by TNFa stimulation for another 3 hours. The total RNA was extracted by miRNeasy mini Kits (Qiagen) following the manufacture instruction. The transcriptome profiling of keratinocytes was performed using Affymetrix Clariom S microarray.