SMASH – semi-automatic muscle analysis using segmentation of histology: a MATLAB application

SMASH is composed of a set of functions enabling the user analyze aspects of histological muscle sections. Image segmentation: identifies potential muscle fibers. Fiber filter: removes non fiber elements. Fiber properties computes parameters related to fiber size. Fiber type: determines which fibers are positively stained within an image. Central nucleation: determines which fibers have central nuclei within an image. Object counter: identifies non fiber objects within an image. SMASH Stand Alone requires MATLAB Compiler Runtime (MCR) version R2014a (8.3) which is freely available from Mathworks (http://www.mathworks.com/products/compiler/mcr/). SMASH App requires MATLAB version R2014a (8.3) or later with the Image Processing Toolbox.SMASH是一套用于分析组织学肌肉切片相关特征的功能集，包含以下功能模块： 1. 图像分割（Image segmentation）：识别潜在的肌纤维； 2. 纤维筛选（Fiber filter）：剔除非纤维类成分； 3. 纤维属性计算（Fiber properties）：计算与纤维尺寸相关的各类参数； 4. 纤维分型（Fiber type）：判定图像中呈阳性染色的纤维类型； 5. 中央核检测（Central nucleation）：识别图像内带有中央核的纤维； 6. 非纤维对象计数（Object counter）：统计图像中的非纤维对象。 独立版SMASH需搭配MATLAB运行时环境（MATLAB Compiler Runtime, MCR）R2014a（8.3）版本，该资源可从MathWorks官网免费获取（http://www.mathworks.com/products/compiler/mcr/）。应用版SMASH则需搭载R2014a（8.3）及更高版本的MATLAB，并配备图像处理工具箱（Image Processing Toolbox）。