File comprising the raw values used to create the figures present in the publication "Bioinspired Collagen/Hyaluronic Acid/Fibrin-Based Hydrogels for Soft Tissue Engineering: Design, Synthesis, and In Vitro Characterization"

The dataset submitted contains the raw analytical data, after being treated, used to create the graphs for the following analysis:Figure 2b - degree of Crosslinking obtained from the fluorescence measurements after a TNBS assay which assessed the amount of free amines in a molecule, in this case, collagen. Values were then reported as percentage of free amines. Figure 2c - Rheological data showing the viscoelastic properties of the produced hydrogel materials, depending on the ratio and concentration of crosslinker, PEG.Figure 2d - Rheological data showing the viscosity variation according to different shear rates to demonstrate how the material deforms and assess its suitability for bioprinting. Figure 3 - Rheological data showing the viscoelastic properties of the selected hydrogel formulation, CHAF_1, with time (a), upon different strains (deformation) (b), and stiffness (c).Figure 4b - data collected from the spectroscopy analysis (FTIR) on the raw materials and final hydrogel to study the molecular conformation.Figure 5b - Measurement of how much water is taken by the dry material, based on weight changes, converted into percentage.Figure 6b - Values of pore diameter obtained from manual measurement of pores from images obtained via scanning electron microscopy. Data is arranged as a distribution graph.Figure 6d - Measurement of how much weight is loss for the samples immersed in physiological conditions ( weight changes converted into percentage).Figure 7b - Fluorescence measurement of the data obtained from a presto blue assay, which studies the metabolic activity of the cell-seeded onto the hydrogel and control plastic.Figure 8a,b - Fluorescence measurement of the data obtained from a presto blue assay, which studies the metabolic activity of the cell-seeded into the hydrogel and control plastic.Figure 9b - Values obtained for the area of spheroids measured from micrographs taken with an optical microscope. Area monitored across 7 days.Figure 9d - Cell viability using a measurement of green fluorescence.

提交的数据集包含经过处理的原始分析数据，这些数据被用于生成以下分析的图表：图2b - 通过TNBS检测获得的胶原蛋白分子中游离胺的交联度，经荧光测量后以游离胺的百分比形式报告。图2c - 表现出不同交联剂和PEG比例及浓度的水凝胶材料的粘弹性能的流变学数据。图2d - 根据不同的剪切速率变化的粘度数据，展示了材料的变形情况，并评估其生物打印的适用性。图3 - 展示所选水凝胶配方CHAF_1的粘弹性能随时间（a）、不同应变（变形）（b）和刚度（c）变化的流变学数据。图4b - 对原始材料和最终水凝胶进行的傅里叶变换红外光谱分析（FTIR）收集的数据，用于研究分子构型。图5b - 根据重量变化测量干燥材料吸收的水分，转换为百分比。图6b - 通过扫描电子显微镜获得的图像中孔的手动测量得到的孔径值，数据以分布图形式排列。图6d - 在生理条件下浸泡的样品重量损失测量，重量变化转换为百分比。图7b - 通过presto blue实验获得的数据的荧光测量，研究接种于水凝胶和控制塑料上的细胞的代谢活性。图8a,b - 通过presto blue实验获得的数据的荧光测量，研究接种入水凝胶和控制塑料中的细胞的代谢活性。图9b - 使用光学显微镜拍摄的显微照片测量的球体面积值。面积监测持续7天。图9d - 通过绿色荧光测量评估的细胞活性。