A global blueberry phylogeny: Evolution, diversification, and biogeography of the tribe Vaccinieae (Ericaceae)

Vaccinieae is a morphologically diverse and species-rich (~1440 species) tribe in Ericaceae, itself a clade of ~4600 species. Although the majority of diversity is tropical, Vaccinieae are best known for temperate crops (i.e., blueberries, cranberries, huckleberries, lingonberries) in Vaccinium. Vaccinium itself (~500 species) has been previously suggested as highly polyphyletic, and taxonomic boundaries among many of the other genera in the tribe remain uncertain. We assessed the evolutionary history of Vaccinieae with phylogenomic analyses based on a target-enrichment dataset containing 353 low-copy nuclear gene regions and over 200 taxa representing 30 of the 34 genera in the tribe, and 25 of the 29 sections of Vaccinium. A plastome dataset for a subset of these taxa was additionally constructed. We conducted time-calibrated biogeographic analyses and diversification analyses to explore the area of origin and global dispersal history of the tribe. The analysis recovered a temperate N..., DNA sequence data were derived from three sources. The DNA of most samples was extracted from herbarium specimens with the SLIMS method, a system connecting sampling and wet lab methodology (Folk et al., 2021). Specimens were sampled from the following herbaria (acronyms as in Thiers, updated continuously): FLAS, K, MICH, MO, and NY. Some DNAs (those from PWF) were from silica gel-dried leaf material. The DNA of these samples was extracted with DNeasy®. We quantified DNA amounts of these samples using a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, California, USA) with the Qubit dsDNA Broad Range Assay Kit as per manufacturer recommendations. Finally, some sequence data were downloaded from the Kew DNA Bank.
Library preparation and target capture were performed at Rapid Genomics (Gainesville, FL, USA) with the Angiosperms353 v1 target capture kit (Johnson et al., 2019) to obtain 353 putatively single-copy nuclear loci from each sample. DNA sequencing was conducted on Illumina sequencing..., , # A global blueberry phylogeny: Evolution, diversification, and biogeography of the tribe Vaccinieae (Ericaceae)

[https://doi.org/10.5061/dryad.ksn02v7cr](https://doi.org/10.5061/dryad.ksn02v7cr)

## Description of the data and file structure

These are alignment files (.fasta) of sequences that have already been through a filtering process (see methods), that we used to generate the main topologies in our study with IQtree. This submission includes (1) the nuclear dataset of 262 samples of 353 nuclear loci, and (2) the other is 78 of the plastome for 122 species. 

## Sharing/Access information

The data, novel scripts, and output files that support the findings of this study are openly available in supplementary materials to the related article. Raw sequence reads are available from NCBI PRNA839108, PRJEB49299, and PRJEB51566.
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