Analysis of reverse transcription by wild type or RT-W252A mutant virus in Jurkat cells.

1. The infection experiments were repeated at least three times and the mean copy numbers of DNA measured and standard deviations are shown;2. HIV-1NL4.3;3. HIV-1NL4.3-RT-W252A;4. Heat inactivated virus samples measured less than 100 copies (subtracted);5. The data sets analyzed were considered to be normal with an α value of > 0.05 standard deviations. P values from Welch’s two-tailed t test are shown, comparing experimental and control samples as indicated;6. Nevirapine (NVP) was added at 40 nM which is the reported IC50 in cell culture;7. NT = Not Tested;8. CA measured by ELISA;9. RT measured by in vitro RT assay.Analysis of reverse transcription by wild type or RT-W252A mutant virus in Jurkat cells.