Septic shock patients: outcome at D1 (24 hours after inclusion). Homo sapiens

Microarray was performed on PBMC with an inflammatory dedicated slide of 340 genes, and target samples labelled with Cy3 for day 0 (reference) and with Cy5 for the other days. Changes in expression levels were evaluated by the ratio Dx/D0. According to outcome and time evolution, a two-dimensional clustering was performed. Keywords: dead vs alive septic shock patients at D1 post-inclusion Overall design: The day of inclusion corresponded to the first blood sampling (15 ml) quoted day 0. The following blood samples were performed at days 1, 7, and 28. Since the pre-sepsis expression of the genes as a control cannot be obtained, the day 0 sample was used patient per patient as a reference value for the next days samples for micro-array study. Patients outcome (dead or alive of evolution) was considered, as the time evolution when the patients survived.