Macroconidiation in ΔFvhtf1 mutant.

(A) In wild-type strain (A149) and the complementation strain (ΔFvht1-Com), incipient macroconidia without septation are produced on terminal phialides or hyphae. The gene-deletion mutant (ΔFvhtf1) failed to form macroconidia from aberrant terminal phialides. ΔFvhtf1 produced mature conidium with clear septation from the tip of the hyphae. The macroconidia of ΔFvhtf1 deletion mutant showed a morphological defect. These observed phenotypes in ΔFvhtf1 are consistent with what we witnessed in F. graminearum mutant (ΔFghtf1). tp, terminal phialides; ma, macroconidia; nr, narrow region, ac, apical cell; bc, basal cell (foot cell). Bar = 20 µm. (B) Macroconidia production by A149, ΔFvhtf1, and ΔFvht1-Com in mung bean liquid medium under continuous dark conditions. (C) Macroconidia production by A149, ΔFvhtf1, and ΔFvht1-Com in mung bean liquid medium under UV light conditions.