RNA_sequencing_AF_right_atrium_2018

Total RNA including small RNAs was extracted from RA biopsies of six AF patients and five controls. Tissue samples were homogenized in QIAzol reagent, DNase treated and RNAs were isolated using the miRNeasy kit (QIAGEN, Hilden, Germany) according to manufacturer’s instructions. Ribosomal RNAs were depleted from 50ng of total RNA using the Ribo-Zero rRNA removal kit (Illumina, San Diego, California, USA). Barcoded cDNA libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, California, USA) following manufacturer’s protocol. Libraries were then sequenced on an Illumina HiSeq 2500 with four library samples per lane (125 bp pair-end reads). The average number of reads per sample ranged from 71 to 82 million reads.