The data underlying Fig 6.

(A) Destabilizing effect of SPT inhibition on inclusions. GFP10-expressing HeLa cells were infected with the indicated strains and parallelly treated with MYR or LCS or left untreated (control). Cells were fixed, stained, and imaged at the indicated time points. The percentage of infected cells, surviving cells, and infected cells containing cytosolic bacteria was determined by manual image analysis. Data are displayed in Fig 6A. (B) Inclusion-destabilizing effects of SPTLC1 depletion or KDSR deficiency. The indicated cell lines were transfected with a GFP1–10-expressing plasmid and then infected with the indicated strains. Cells were fixed, stained, and imaged at the indicated time points. The percentage of infected cells, surviving cells, and infected cells containing cytosolic bacteria was determined by manual image analysis. Data are displayed in Fig 6C. (C) Treatment with SKI-II destabilizes CTL2 inclusions. GFP10-expressing HeLa cells were infected with the indicated strains and parallelly treated with SKI-II or solvent-only (DMSO). Cells were fixed at 24 hpi, stained, and imaged. The percentage of infected cells displaying inclusion damage was determined by manual image analysis. Data are displayed in Fig 6D. (XLSX)