Proximity labeling of autophagic proteins to investigate the Arabidopsis thaliana autophagy regulatory network

In order to explore the regulatory network of plant autophagy, this study generated stable transgenic lines expressing ATG5-TurboID, ATG7-TurboID and ATG11-TurboID. Autophagy was induced by combined treatment with the autophagy inducer AZD8055 and biotin. Proximal proteins of autophagy-related proteins under autophagic conditions were enriched using immunoprecipitation coupled with mass spectrometry (IP-MS), followed by network analysis to identify potential interacting proteins and functional modules. This study provides a richer range of candidate targets for further dissecting the autophagy regulatory network in Arabidopsis thaliana.