Dissociation of the GpA TM domain in APol upon addition of SDS.

Steady-state FRET measurements (n = 3) were performed at a fixed polymer (20 µM) to peptide (0.5 µM) ratio (40∶1). SDS was added to preformed APol/GpA complexes, and emission spectra were recorded at 25°C after incubation at 37°C. (A) Dimer fractions of the GpA TM domain determined by FRET efficiencies of the fluorescence emissions spectra plotted against SDS concentration (see eq. 3). (B) Logarithm of the apparent GpA TM dissociation constants at increasing SDS concentration (see eq. 4).