Interstitial Biofilm Formation in P. Aeruginosa (data for Figure S5-lower left panel in related publication) - time series images

This folder contains 1000 tiff images. These tif images comprise a time series captured at a rate of 1 frame every 2 seconds. These images were captured using an inverted microscope with a 100x phase oil-immersion objective (Olympus IX-71) fitted with an FViewII (Soft Imaging Systems) monochromatic camera.

This movie is associated with the lower left panel of Figure S5 in the related publication. Analysis was done using the protocol described in the related publication (Supplementary 1).

Interstitial biofilms of the bacteria Pseudomonas aeruginosa were cultured on 0.4xLB-Lennox solidified with 8 g/L gellan gum (twitching motility gellan gum, TMGG). Molten TMGG was poured over sterile slides and solidified at room temperature. Slides were inoculated, covered with a sterile coverslip, and incubated in humid conditions at 37 °C. Media was supplemented with 100 Kunitz units per mL DNaseI (D5025, Sigma Aldrich) or the enzyme storage buffer [50% vol/vol glycerol, 10 mM MgCl2, 10 mM CaCl2, 10 mM Tris•HCl].

本文件夹包含1000张TIFF图像。该组TIF图像构成一套时间序列，采集帧率为每2秒1帧。本次成像使用搭载FViewII（Soft Imaging Systems）单色相机的奥林巴斯IX-71倒置显微镜，搭配100×相位油浸物镜。

该影像对应已发表相关文献中补充图S5的左下面板。所有分析均遵循该文献记载的实验方案（补充材料1）。

实验样本为铜绿假单胞菌（*Pseudomonas aeruginosa*）的间隙生物膜，培养于以8g/L结冷胶固化的0.4×LB-Lennox固体培养基（该培养基用于细菌抽搐运动实验时称为结冷胶运动培养基，TMGG）。将熔融态TMGG培养基倾倒至无菌载玻片上，于室温下固化。随后对载玻片进行接种，加盖无菌盖玻片，并置于37℃、湿润环境中孵育。培养基中添加了终浓度为每毫升100昆茨单位的脱氧核糖核酸酶I（DNase I，货号D5025，Sigma Aldrich），或仅添加酶储存缓冲液[体积比50%甘油、10 mM MgCl2、10 mM CaCl2、10 mM Tris•HCl]。