FGFR3 and TAK1 alter gene expression in Bladder Cancer cells.

A microarray experiment was performed using MGHU3 bladder cancer cells transfected with control or TAK1 siRNA, then treated with or without FGFR inhibitor, PD173074. One way ANOVA with fold change magnitude >2 and a p-value with FDR <0.05 was used to generate gene lists. Lists compared samples transfected with control verses TAK1 siRNA and samples transfected with control versus TAK1 siRNA that were additionally treated with PD173074. Genes common to both comparisons were then removed from the control versus TAK1 siRNA list, and are reflected in the table above. Asterisks indicate further validation done by qPCR in separate experiments using MGHU3 cells or KMS11 MM cells treated as in the original microarray experiment. For TNFAIP3, validation in MM cells was dependent on ligand presence. Expression of GSTA1 in MM cells was not detectable.