Alkalinization-defective C. glabrata mutants.

Listed are mutants that showed reduced in vitro alkalinization of phenol red containing YNB medium with 1% casamino acids as sole carbon and nitrogen source in a screen of 647 mutants. Alkalinization defects were verified in independent assays and with two independent clones.A+reduced alkalinization (same phenotype as bcy1Δ in Fig. 5A), ++ strongly reduced alkalinization (same phenotype as put3Δ in Fig. 5A) as compared to the wild type.B, CGrowth was monitored in parallel in YPD and in YNB medium with 1% casamino acids without phenol red by measuring absorption at 600 nm. ++ strong growth defect, + weak growth defect, - unaltered growth as compared to the wild type.DMutants were co-incubated with MDMs for 90 min and phagosome acidification was monitored by LysoTracker staining. At least three independent microscopic fields were scored per mutant. ++ strong increase in LysoTracker signal, + medium increase in LysoTracker signal, - no change in LysoTracker signal as compared to the wild type.