Supplementary material from: Deep divergences among inconspicuously colored clades of Epipedobates poison frogs

Please see López-Hervas et al. (2024) for the full methods. In this study, we evaluate and clarify the phylogeny of the dendrobatid poison frog genus Epipedobates. Although Epipedobates is considered one of the youngest clades in the Dendrobatidae family, we find tremendous phenotypic variation within and among species, making them an interesting group for taxonomists and evolutionary biologists. We aimed to comprehensively assess the phylogenetic relationships among species within this clade, which until now has remained incomplete despite several in-depth studies. Briefly, we sequenced mtDNA and nDNA and estimated phylogenetic trees using Bayesian and Maximum Likelihood approaches (see Data S1, Fig. S1, Fig. S2, Table S2). We estimated and reviewed haplotypes based on each sequenced gene (Table S3). We then used alignments of mtDNA to estimate putative species limits in 1) Assemble Species by Automatic Partitioning (ASAP) and 2) Generalized Mixed Yule Coalescent (GYMC) (see Data S2 and Fig. S3). We reviewed published specimen data to estimate species ranges and differences in body sizes among putative species (see Data S3). Finally, we reviewed images of all examined specimens (Fig. S4-S27) as well as accompanying metadata (Table S1) to qualitatively compare color among species.