Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [NKT_after_Dex]

The goal of this study was to understand the role of thymic natural killer T cells (NKT) in thymic regeneration following dexamethasone-induced acute involution. For this purpose we treated WT mice with dexamethasone, sorted NKT from their thymi 3 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed bulk RNA Seq. Young male WT mice were treated with dexamethasone 3 days/18 hours before sacrifice, and then thymic NKT were isolated and mRNA profiles were generated by deep sequencing using the MARSseq method. 4-5 replicates were used. The control was 3 days vehicle-treated age-matched WT mice.