High-content imaging data

# Scope

These are microscopy image datasets of a a primary screen and two secondary siRNA screens for histone regulators in the breast cancer cell line MDA-MD-231.

The datset is part of an article by Bruhn et al., 2022 (Communications Biology).

# Associated sample information

For each screen, a separate sample map is provided in tab-separated format.

The "dataset" column designates the folder in which the associated data are stored.

Other columns indicate the stainings (channels), the siRNA targets.

# Folder and file structure

Each of the 3 screening folders contains one subfolder per screening plate.

Each plate folder contains one subfolder per column group that was acquired within a single run.

Each column group folder contains one subfolder per optical field with the nomenclature OPTICALFIELD_WELL

Each OPTICALFIELD_WELL folder contains one subfolder per channel

Each channel folder contains a subfolder "stack1" in which the image is stored in ets format (frame_t_0.ets)

# Additional information

Detailed technical information on sample preparation and image evaluation can be found in the associated article.