Transcriptomic profile of cystic fibrosis airway epithelial cells undergoing repair

The purpose of the study is to compare the transcriptomic profile of the airway epithelium generated from bronchial airway epithelial cells isolated from healthy donors (NCF) and patients with cystic fibrosis (CF). Cells were grown at the air-liquid interface for at least 2-months. CF is caused by mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Only patients homozygous for the F508del mutation of the CFTR gene were considered. The reconstituted airway epithelium was mechanically wounded and allowed to repair with time. We considered four steps: 1) intact, non-wounded (NW) epithelium; 2) 24h hours post-wounding (pW); 3) time at which the wound is closed (WC); 4) two days post-wound closure (pWC). We also mimicked infection by exposing the cells to Pseudominas aeruginosa flagelin for NW and WC conditions.

本研究旨在对比健康供体（healthy donors, NCF）与囊性纤维化（cystic fibrosis, CF）患者分离的支气管气道上皮细胞所构建的气道上皮的转录组谱。细胞于气液界面（air-liquid interface）培养至少2个月。囊性纤维化由囊性纤维化跨膜传导调节因子（cystic fibrosis transmembrane conductance regulator, CFTR）基因的突变引发，本研究仅纳入携带CFTR基因F508del突变纯合子的患者。随后对重构的气道上皮实施机械损伤，并随时间观测其修复过程。本研究设定四个观测阶段：1）完整未损伤（non-wounded, NW）上皮；2）损伤后24小时（post-wounding, pW）；3）伤口闭合（wound closure, WC）时刻；4）伤口闭合后两天（post-wound closure, pWC）。此外，我们还在未损伤及伤口闭合状态下，将细胞暴露于铜绿假单胞菌（Pseudomonas aeruginosa）鞭毛蛋白（flagelin）以模拟感染。

搜集汇总

背景与挑战

背景概述

该数据集是一个人类转录组学研究，专注于比较健康捐赠者和囊性纤维化患者的气道上皮细胞在修复过程中的基因表达变化。数据集包含78个样本，使用RNA-Seq技术，并模拟了创伤修复和细菌感染条件，旨在揭示囊性纤维化相关的分子机制。