Stage-Specific Proteomic and Phosphoproteomic Insights into Theileria annulata: Molecular Adaptations in Bovine Schizont and Merozoite Stages

In the present experiments, we used in vitro cultured bovine lymphocytes infected with T. annulata to purify the cycloartilaginous stage shizonts of T. annulata and the cycloartilaginous stage merozoites of T. annulata after using T. annulata-infected erythrocytes from live cows (with 30% staining rate), and then used the Proteomics and phosphorylated proteomics data of the two stages of T. annulata were measured on a Thermo Orbitrap Astral mass spectrometer for mass spectrometry uptake and resolution.