Transcriptomic analysis of DNA damage response in zebrafish embryos under simulated microgravity

The dataset for this project was taken from the study titled “RNA sequencing of zebrafish embryos under simulated microgravity conditions (GLDS-373)” submitted to the NASA GeneLab repository by the authors. The accession number of the project is GSE145967. We downloaded the RNA-SEQ raw gene counts data of 12 wild-type AB Zebrafish (Danio rerio) of both sexes, 3–12 months of age and approximately 3–4 cm in body length. They were constantly subjected to a 12/12h light/dark cycle and maintained in a circulating water bath at 28.5°C under standard laboratory conditions. The zebrafish embryos were produced through natural breeding conditions with autoclaved system egg water containing 60μg/mL sea salt in distilled water according to the previously reported protocols.