Transcriptomic analysis of DNA damage response in zebrafish embryos under simulated microgravity

The dataset for this project was taken from the study titled “RNA sequencing of zebrafish embryos under simulated microgravity conditions (GLDS-373)” submitted to the NASA GeneLab repository by the authors. The accession number of the project is GSE145967. We downloaded the RNA-SEQ raw gene counts data of 12 wild-type AB Zebrafish (Danio rerio) of both sexes, 3–12 months of age and approximately 3–4 cm in body length. They were constantly subjected to a 12/12h light/dark cycle and maintained in a circulating water bath at 28.5°C under standard laboratory conditions. The zebrafish embryos were produced through natural breeding conditions with autoclaved system egg water containing 60μg/mL sea salt in distilled water according to the previously reported protocols.本项目所用数据集取自作者提交至NASA GeneLab数据库的题为“模拟微重力条件下斑马鱼胚胎的RNA测序（GLDS-373）”的研究。本项目的登录号为GSE145967。我们下载了12尾雌雄兼具、3~12月龄、体长约3~4cm的野生型AB品系斑马鱼（Danio rerio）的RNA测序原始基因计数数据。该批斑马鱼始终处于12/12小时的光暗循环环境中，于28.5℃的循环水浴中按标准实验室条件饲养。斑马鱼胚胎通过自然繁育获得，繁育所用的系统卵水经高压灭菌处理，其成分为蒸馏水中添加60μg/mL海盐，制备流程遵循此前已发表的实验方案。