Multiregional Glioma Single-Cell Transcriptomic Sequencing

Glioma is a complex malignant neoplasm, treating each case as a dark box obviously could not acquire expected overall prognosis, because subpopulation inside tumor could be core factor which directly influence tumor progression. So, clonal sequencing or single-cell sequencing could be best solution to this problem. Here we combined these two strategies and analyzed more than 6000 single-cell transcriptome of 73 surgery points from 13 glioma and 1 brain metastasis patient. After these cells assigned into normal, immune, tumor and several other groups based on differentially expressed genes and specified TCGA 4 classification gene sets, spacial information of sampling points helped us built up cell type constituent of different part inside tumor, and illustrated invasion patterns during tumor growth. Here we combined these two strategies and analyzed more than 6000 single-cell transcriptome of 73 surgery points from 13 glioma and 1 brain metastasis patient