Error-corrected Duplex Sequencing of TK6 cells exposed to 4-nitroquinoline 1-oxide

Error-corrected Duplex Sequencing (DuplexSeq) can correct errors introduced during DNA sample handling and PCR amplification through generating consensus sequences by labeling each strand of a DNA duplex with a unique molecular identifier. This error correction can decrease the error rate of conventional NGS by approximately 10,000x. The DuplexSeq human mutagenicity panel contains 20 2.4-kb target regions across 20 autosomal chromosomes. The panel is representative of the whole genome in terms of % GC-content and relative proportions of coding and non-coding regions and can be used to measure mutation frequencies (MF) in biological samples or cultured cells. In this study, we applied DuplexSeq to quantify induced mutation frequency and to measure the mutation spectrum in human lymphoblastoid TK6 cells exposed to a reactive oxygen species (ROS)-inducing compound, 4-nitroquinoline 1-oxide (4NQO).