Splenocyte and Naïve CD4+ T cell proliferation in response to C.difficile-stimulated BMDCs.
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WT BMDCs were infected with PFA-fixed C. difficile strains at an MOI of 50. 24 h post-infection, infected BMDCs were co-cultured with CFSE-labelled splenocytes in the presence of anti-CD3/CD28. Proliferation of CD4+ gated cells 96 h post stimulation (A). Quantification presented as percentage of proliferating cells (B). Infected BMDCs were co-cultured with CFSE-labelled naïve CD4+ T cells from OT-II transgenic mice in the presence of OVA323–339 and T cell proliferation was analysed (C). 1 µg/ml LPS utilised as a reference stimulus. ***p
创建时间：
2016-02-24
