P7 Kdr-KO scRNA seq data
收藏Figshare2025-07-23 更新2026-04-08 收录
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https://figshare.com/articles/dataset/P7_Kdr-KO_scRNA_seq_data/29626994/1
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资源简介:
Lung tissue from four experimental groups Kdr<sup>flox/flox</sup>and Tbx2<sup>icre</sup>Kdr<sup>flox/flox</sup>under maintained in normoxia or exposed to chronic hyperoxia were euthanized at P7. Lung tissue was dissected and snap-frozen in liquid nitrogen. Nuclei were prepared from frozen tissue using a nuclear isolation kit from 10X Genomics (10x Genomics, PN-1000494). For 10X Chromium, 10,000 nuclei were loaded per lane. The libraries were prepared according to manufacturer protocol (10X Genomics) using 3’V3 Kits (10X Genomics, PN-1000269) and were submitted for sequencing via a NovaSeq 6000 flow cell to a depth of 50,000 reads/cell. Raw sequencing data were processed using CellRanger pipeline
提供机构:
Knutsen, Carsten
创建时间:
2025-07-23



