Transcription profiling by high throughput sequencing of mouse brain, heart, kidney, liver, lung, skeletal muscle organ, spleen and testis

As part of the ENCODE consortium the GENCODE project is producing a reference gene set through manual and automated gene prediction. Selected transcript models are verified experimentally by RT-PCR amplification of at least one of their unique splice junctions followed by high-throughput sequencing. The experiment targets are manually annotated transcripts with novel or putative status, non-pseudogene biotype and unique splice junctions which have not been validated previously and are not supported by mouse RNAseq data from the ENCODE project. In Batch M2, 808 splice junctions from GENCODE version M4 (released in December 2014) were chosen for experimental verification.作为ENCODE（Encyclopedia of DNA Elements）联盟的组成部分，GENCODE项目正通过人工与自动化基因预测手段构建参考基因集。筛选得到的转录本模型，将通过至少针对其一条独特剪接接头的RT-PCR（逆转录聚合酶链式反应）扩增结合高通量测序的方式完成实验验证。本实验的靶标为经人工注释、具备新型或推定状态、非假基因生物型，且此前未被验证、亦未得到ENCODE项目小鼠RNA测序数据支持的转录本。在M2批次中，研究人员选取了GENCODE M4版（2014年12月发布）中的808个剪接接头用于实验验证。