Data related to: Layer 4 fast-spiking interneurons filter thalamocortical signals during active somatosensation. Yu, Gutnisky, Hires, Svoboda, Nature Neurosci. 2016.

These experiments measure spikes and membrane potential in the mouse somatosensory
thalamocortical system (Yu, Gutnisky et al Nat. Neurosci. 2016). Mice performed an active
object localizatiobn behavior (as in O’Connor, D JNS 2010). Importantly, whisker movements
and touch forces were tracked with millisecond time-scale precision (as in Pammer et al JNS 2013;
Hires, Gutnisky et al ELife 2015). Spikes were recorded in VPM, L4 excitatory neurons, and L4
fast-spiking, parvalbumin-expressing interneurons. Importantly, L4 recordings were made with
loos-seal pipettes to avoid sampling bias. Membrane potential is in addition recorded in L4 excitatory
neurons and L4 fast-spiking neurons. Various optogenetic manipulations were performed to identify
neuron types and to analyze the flow of excitation in the circuit.