Leveraging environmental DNA (eDNA) to optimize targeted removal of invasive fishes
收藏资源简介：
Natural resource managers need innovative and cost-effective methodologies that enable the targeted removal of aquatic invasive species (AIS). Removing AIS before they establish and spread into critical habitat for native species can mitigate invasions in freshwater systems and preserve ecosystem integrity. To address this need, we established protocols using the detection of environmental DNA (eDNA) to guide deployment of traditional fisheries trapping methods for invasive fish species removal. In a pilot study during spring 2022, we set minnow traps (one per site) in a spatially stratified random design. We also developed a quantitative polymerase chain reaction (qPCR) assay to detect eDNA from multiple closely related invasive loach species (family Cobitidae) and compared detections of eDNA with detections of fish using minnow traps. At sites where both eDNA and minnow traps were deployed, the two methods agreed on the presence of loaches approximately 79% of the time (95% CI: 60%-90..., See briefly summarized methods in the associated ReadMe file of this dataset. For more detailed methods see methods described in main manuscript., , # Data from: Leveraging Environmental DNA (eDNA) to Optimize Targeted Removal of Invasive Fishes \[[https://doi.org/10.5061/dryad.n8pk0p342](https://doi.org/10.5061/dryad.n8pk0p342)] ## General information ### Author Information Corresponding author: Jennie J. Wiggins Fish Biologist, Lodi Fish and Wildlife Office US Fish and Wildlife Service [jennie_wiggins@fws.gov](mailto:jennie_wiggins@fws.gov) Co-Authors: Vanessa D. Tobias Mathematical Statistician, Lodi Fish and Wildlife Office US Fish and Wildlife Service [vanessa_tobias@fws.gov](mailto:vanessa_tobias@fws.gov) Erika F. Holcombe Supervisory Fish Biologist, Lodi Fish and Wildlife Office US Fish and Wildlife Service [erika_holcombe@fws.gov](mailto:erika_holcombe@fws.gov) Katie Karpenko Research Associate, Genidaqs Laboratory Cramer Fish Sciences [katie.karpenko@fishsciences.net](mailto:katie.karpenko@fishsciences.net) Eric R. Huber Supervisory Fish Biologist, Lodi Fish and Wildlife Office US Fish and Wildlife Service [eric_hub...
