STAT5 inhibition abrogate TSLP-induced homeostatic maturation of human cDC2s

It has been reported that TSLP activates several downstream signalings including STAT5. To test whether TSLP triggers the homeostatic maturation program of human splenic cDC2 (DC2hm program) in a STAT5-dependant manner, we administrated TSLP to in-culture human splenic cDC2 with or without STAT5 inhibitor and performed RNA-seq. The results showed that TSLP-induced DC2hm-like profile was abrogated by STAT5 inhibition. Overall design: Purified cDC2s were treated with TSLP (50 ng/ml) without the presence of STAT5 inhibitor (100µM) for 12 hours. UID RNA-seq which incorporating unique identifiers to improve sensitivity and remove PCR-ampliaction bias was then performed on TSLP-cDC2s, Med-cDC2s and purified cDC2s.