Identifying Key Metabolites Associated with Glucosinolate Biosynthesis in Response to Nitrogen Management Strategies in Two Rapeseed (Brassica napus) Varieties

A high glucosinolate (GSL) concentration, an undesirable substance, has severely restricted rapeseed (Brassica species) development. We performed widely targeted metabolomics analysis based on the ultra-high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (UPLC–ESI–MS/MS) technology to analyze the metabolic profiles and identify the differential metabolites and GSL components in response to different nitrogen (N) levels in two rapeseed varieties. A total of 341 metabolites and 38 GSL components were detected in the seeds. A total of 188 differential metabolites, including 34 GSL components, were identified in response to different treatments, which were mapped into 2-oxocarboxylic acid metabolism, tryptophan metabolism, and GSL biosynthesis. Key indicators of GSL components highly responsible for different N levels under two contrasting varieties were recognized, i.e., 1-methylpropyl GSL and 4-methylthiobutyl GSL. This study suggests that the efficient N management and variety selection are important strategies for developing rapeseed with low GSLs.