Effect of GDM and insulin on hENT1 and hENT2 relative contribution to total adenosine transport.

hPMEC from normal (N) or gestational diabetes (GDM) pregnancies were cultured (8 hours) in the presence of basal levels of insulin (C) or 1 nmol/L insulin (Ins) and kinetic parameters for hENT1- or hENT2-mediated adenosine transport (i.e., maximal velocity (Vmax) and apparent Michaelis-Menten constant of saturable transport (Km)) measured as described in Methods. The Vmax and apparent Km values were used for estimation of F values (see Methods).