Data Sheet 1_Predictive value of circulating SNHG1/miR-194-5p and carotid ultrasound for the onset of cerebral infarction in patients with transient cerebral ischemia.xlsx

BackgroundTransient cerebral ischemia is a strong warning sign of cerebral infarction (CI). Early objective risk assessment in patients with transient cerebral ischemia can effectively help prevent the occurrence of CI.
ObjectiveThe study aimed to explore the predictive value of SNHG1/miR-194-5p in combination with carotid ultrasound for predicting the occurrence of CI in patients with transient cerebral ischemia.
Patients and methodsThis study was a prospective observational study. A total of 189 patients with transient cerebral ischemia were included and divided into the CI group (n = 67) and the non-CI group (n = 122) based on whether CI occurred within 90 days. The clinical data and laboratory indexes of the two groups were compared. RT-qPCR was employed to examine the levels of SNHG1/miR-194-5p. Logistic regression analysis and receiver operating characteristic (ROC) curve analysis were performed based on serum SNHG1/ miR-194-5p levels and the degree of carotid artery stenosis. In addition, bioinformatics analysis was carried out to identify the target genes of miR-194-5p.
ResultsThe results showed that, compared to the non-CI group, the expression of SNHG1 in the serum of the CI group was upregulated, while the expression of miR-194-5p was downregulated. Logistic regression analysis showed that the expression of miR-194-5p (OR = 0.067, p < 0.001) and SNHG1 (OR = 25.984, p < 0.001) and the degree of carotid artery stenosis (OR = 1.152, p = 0.001) were significantly correlated with CI. The combined detection of these three indicators yielded an AUC value of 0.953 for predicting CI. Its sensitivity was 89.55% and specificity was 86.89%, indicating higher diagnostic efficiency than any single indicator. Furthermore, bioinformatics analysis revealed that the target gene of miR-194-5p was enriched in various disease pathways, especially those related to neurodegeneration, providing a new direction for exploring the mechanism of CI.
ConclusionSerum SNHG1/miR-194-5p levels combined with carotid ultrasound show high predictive accuracy for the short-term occurrence of CI in patients with transient cerebral ischemia.