five

pNB90 backbone for the genomic library screen.

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Figshare2016-02-23 更新2026-04-29 收录
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Random size-selected genomic fragments were cloned 5’ to ‘phoA to create a library that could be screened to detect sequences capable of mediating secretion of PhoA. pBBR1 ori = origin of replication; nptII = neomycin phosphotransferase II conferring kanamycin resistance; ‘phoA = E. coli alkaline phosphatase gene with no native signal sequence.
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2016-02-23
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