CAB-DMF: A Capillary Action-Based Digital Microfluidic Sampling Method for Enhanced Precision in Trace-Level Omics Analysis

We report a robust CAB-DMF sampling method aimed at optimizing the application of DMF platforms in trace-level omics analysis. Specifically, we propose a method for extracting nano-liter scale samples from DMF chips using capillary action, without the need for external force assistance, along with a complementary sample drainage device. Finally, we demonstrated the capability of the capillary sampling method in single-cell proteomics analysis based on DMF platforms.