Richard Allen (University of Hawaii) (2011) CIL:9854, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

A median longitudinal section through this collecting canal that may be artificially swollen at two sites where it is encircled by structural microtubules. Smooth spongiome forms a mesh around the collecting canal and these microtubules. Decorated tubule bundles of the decorated spongiome are sparsely scattered peripheral to the smooth spongiome. TEM taken on 7/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5 µm. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:38690) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

本数据集展示了一组此收集管的中位纵向横截面，该横截面在两处可能由于被结构性微管环绕而人工膨胀。平滑海绵状结构围绕收集管及其微管形成网状结构。装饰性海绵状结构的装饰性管束稀疏地散布在平滑海绵状结构外围。该透射电子显微镜图像由R. Allen于1996年8月7日使用Zeiss 10A电子显微镜，在80kV的电压下拍摄。负片放大倍数为9,780倍。刻度尺长度为0.5微米。细胞使用0.25%戊二醛轻轻固定，并用2.3M蔗糖渗透处理，之后在液氮中冷冻，并在-100°C的温度下，以约75纳米的厚度进行切片。从这些制备中获得的冷冻切片随后被解冻、清洗，并暴露于由小鼠或兔/山羊制备的单克隆原抗体，以及与胶体金复合的山羊抗小鼠/兔二抗。制备的详细过程见《细胞生物学方法》2010年第96卷第143-173页。负片被打印到纸上，并扫描至Photoshop。此数字化图像可用于定性分析。该图像的未经处理的原始高分辨率版本（CIL:38690）存档于图书馆，可供定量分析。更多详细信息请参阅（http://www5.pbrc.hawaii.edu/allen/）。