Dataset of the package TepR (Transcription Elongation Profile in R)

TepR (Transcription Elongation Profile in R) is an R package designed for analyzing data from nascent RNA sequencing technologies, such as TT-seq, mNET-seq, and PRO-seq. It calculates the probability distribution of nascent RNA sequencing signal across the gene body or transcription unit of a given gene. By comparing this profile to a uniform signal, TepR can identify transcription attenuation sites. Furthermore, it can detect increased or decreased transcription attenuation by comparing profiles across different conditions. Beyond its rigorous statistical testing and high sensitivity, a key strength of TepR is its ability to resolve the elongation pattern of individual genes, including the precise location of the primary attenuation point, when present. This capability allows users to visualize and refine genome-wide aggregated analyses, enabling the robust identification of effects specific to gene subsets. These metrics facilitate comparisons between genes within a condition, across conditions for the same gene, or against a theoretical model of perfect uniform elongation.