E. coli Sacculi +A22 (MG1655)

Tilt Series Date: 2015-04-10 Data Taken By: Matt Swulius Species / Specimen: Escherichia coli Strain: MG1655 sacculi Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: raptor Sample Preparation: Cells were treated for one hour with 10ug/ml A22 until they were rounded and lemon-shaped prior to sacculus purification.

搜集汇总

背景与挑战

背景概述

该数据集包含大肠杆菌MG1655菌株经A22处理后细胞壁的断层扫描原始数据，使用Caltech Polara显微镜在特定倾斜角度和剂量条件下获取。数据包括图片和MRC格式文件，适用于细胞结构研究。