Comprehensive transcriptome analysis of the brain in CNS-specific PRMT1 knockout mice.

Arginine methylation is a common post-translation modification that is catalyzed by protein arginine methyltransferases (PRMTs). We generated the central nervous system-specific PRMT1 deficient (PRMT1-CKO) mice by Cre-loxP recombination system. Recently, we have discovered that PRMT1 is essential for development of CNS, and it is especially crucial for oligodendrocyte lineage progression and the subsequent myelination. In this study, we performed a comprehensive analysis of gene expression changes in whole brains, cortices or cerebella of wild type (WT) and PRMT1-CKO mice using RNA sequencing (RNA-seq). Filtering characteristics were used to identify the differentially expressed genes using the CLC Genomics Workbench software mRNA profiles of mouse brain were generated by RNA sequencing using the NextSeq 500 (Illumina).精氨酸甲基化是一类常见的翻译后修饰，由蛋白质精氨酸甲基转移酶（protein arginine methyltransferases, PRMTs）催化。本研究通过Cre-loxP重组系统构建了中枢神经系统特异性PRMT1敲除（PRMT1-CKO）小鼠。近期研究表明，PRMT1对中枢神经系统（CNS）的发育不可或缺，尤其对少突胶质细胞谱系的分化进程及后续髓鞘形成具有关键调控作用。本研究利用RNA测序（RNA-seq）技术，对野生型（WT）与PRMT1-CKO小鼠的全脑、皮层及小脑组织的基因表达变化进行了全面分析。本研究采用CLC基因组工作台（CLC Genomics Workbench）软件的筛选特征参数鉴定差异表达基因，并通过Illumina NextSeq 500测序平台完成RNA测序，以获取小鼠脑组织的mRNA转录组图谱。