pme-2020-0019 - Supplementary Material: Concentration and integrity indexes of urine cell-free DNA as promising biomarkers for early lung cancer diagnosis

Table 1. Primer and
probe sequences of ALU fragments.

Table 2. Diagnostic
information of ALU fragments in lung cancer patients.

Abstract: Aim: To
explore the role of ucfDNA concentration and integrity indexes as potential
biomarkers for lung cancer diagnosis.

Methods: Quantitative
real-time PCR targeting ALU repeats at three size fragments (ALU-60, 115 and
247 bp) was performed in 55 lung cancer patients and 35 healthy individuals.

Results: ucfDNA concentration
and integrity indexes were significantly higher in lung cancer patients than
in healthy controls. The area under the ROC curve (AUC) for differentiating
patients with stage I/II from healthy controls by ALU fragments concentration
were 0.856, 0.909 and 0.932, respectively. In addition, the ucfDNA integrity
indexes in patients with lymph node metastasis were significantly higher than
in patients with non-metastatic.

Conclusion: ucfDNA concentration and integrity
indexes could serve as promising biomarkers for lung cancer diagnosis.