Cell and molecular transitions during efficient dedifferentiation: population timecourses

Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum, with comparison to forward development RNAseq timecourses sampling the dedifferentiation of 14h developed cells in two different nutrient conditions; liquid growth media (Media, 0 to 18 h) and Klebsiella bacteria (Bacteria, 0 to 24 h), compared with mock dedifferentiation in phosphate buffer (Buffer, 0 to 6 h), a reference sample of undifferentiated cells (Undifferentiated) and a timecourse of forward development (Development, 0 to 14 h). All timecourses performed in duplicate.