Mapping of 2'-O-methylation in Trypanosoma brucei small RNAs using RibOxi-seq

In this study we combined small RNA isolation from post-ribosomal supernatant with RibOxi-seq to identify 2’-O-methylation (Nm) in single nucleotide resolution. Our analysis identified ~49 Nm sites in tRNA, snRNAs, snoRNAs, 7SL RNA and vtRNA. Trypanosoma brucei procyclic form was used to extract small RNAs. The RNA was then underwent RibOxi-seq for 2'-O-methylation profiling protocol with modifications