Effect of carnosine on cytochrome c (Cyt c) contents in mitochondria and cytoplasm in restraint-stressed mice.

Spleen lymphocytes samples containing (2×106) were gently lysed with lysis buffer. Lysates were centrifuged to obtain supernatants (cytosolic extracts free of mitochondria) and the pellets (fraction that contains mitochondria). These two fractions were adjusted to 2 mg protein/ml in the buffer. Contents of cytochrome c were determined using difference spectra at the wavelength pairs of 550∼535, 554∼540 and 563∼577 respectively. The results represent mean ± S.E.M. obtained from 10 animals in each group. The significance of differences from normal group is at##p*p**p