Supplemental data for the manuscript "Effects of a CCR2 antagonist on macrophages and Toll-like receptor 9 expression in a mouse model of diabetic nephropathy"

We
classified kidney macrophages (Mφs) into bone marrow-derived (BM-) macrophages
expressing high CD11b and tissue-specific resident (Res-) macrophages
expressing low CD11b. In diabetic nephropathy (DN) model mice, TLR9 expression
and TNF-α production via TLR9 activation in BM-Mφs and ROS production in
Res-Mφs were enhanced. Furthermore, CCR2 antagonist suppressed the kidney
infiltration of BM-Mφs and their function and the ROS production by Res-Mφs,
with concomitant TLR9 suppression. Our study presents a new therapeutic
strategy for DN.